By Koichiro Yori, Toru Koike (auth.), Masamichi Kamihira, Yoshinori Katakura, Akira Ito (eds.)
Animal telephone know-how is a becoming self-discipline of mobile biology which goals not just to appreciate the constitution, functionality and behaviour of differentiated animal cells, but in addition to examine their skill for use for business and scientific reasons. many of the significant objectives of animal mobilephone know-how contain: the clonal enlargement of differentiated cells, the optimization in their tradition stipulations, modulation in their skill for the creation of medically and pharmaceutically vital proteins and the applying of animal cells to gene treatment, man made organs and practical meals. This quantity supplies the readers an entire evaluate of the current state of the art learn in Japan and different nations the place this box is definitely complex. The court cases may be priceless to cellphone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and to these operating in both educational environments or within the biotechnology and pharmacy industries relating to animal telephone culture.
Read or Download Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 21st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT), Fukuoka, Japan, November 24-27, 2008 PDF
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Additional info for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 21st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT), Fukuoka, Japan, November 24-27, 2008
Pellet diets were prepared by Research Diets (New Brunswick, NJ). Twenty-two mice were used per treatment group. 523 × [length (cm) × width2 (cm2 )] . Immunohistochemical (IHC) determination of steroid hormone-responsive pathway response, proliferation, apoptosis, and angiogenesis were performed as previously described . Prostate specific antigen (PSA), a classic ARG responsive to both androgen and estrogen , was used to assess the effect of resveratrol on steroid hormone-dependent pathways proliferating cell nuclear antigen (PCNA) was used as a proliferation marker.
The results showed that mRNA expression of hexokinase 1 (Hk1) and phosphofrukcutokinase (Pfk) was higher in the KG and RG groups than in the control group. This suggested that the activity of the glycolytic pathway was elevated in the KG and RG groups. On the other hand, the mRNA expression of enzymes related to the TCA cycle, such as Cs, a rate-limiting enzyme, tended to be downregulated in the RG group. These findings suggest that the TCA cycle is not the only metabolic pathway that utilizes the acetyl coenzyme A (CoA) produced by the glycolytic pathway.
Dissected livers were dissolved in TRIzol (Invitrogen) and total RNA was prepared according to the manufacturer’s instructions. RT-PCR was performed as described . Mouse β-actin mRNA was used as the internal control. 5 liver cells were cultured in a Mebiol gel, hepatoblasts rapidly aggregated and formed spheroid structures on day 1 (Fig. 2). Nonparenchymal cells persisted to be dispersed in the gel. With culture, the hepatoblast spheroids enlarged and became compact and smoother on their surface (Fig.