By Claude Desjardins (auth.), Claude Desjardins Ph.D. (eds.)
Conceptual advances within the organic sciences are marked by means of the applica tion of latest suggestions and experimental innovations. Nowhere has this ge neric precept been extra obvious than within the learn of testicular cells, as judged by means of the evolution of issues awarded on the Testis Workshop during the last 23 years. Like its predecessors, the 1995 Testis Workshop was once based to provide clean insights and methods for knowing the mechanisms of spermatogenesis and steroidogenesis. The chapters pre sented during this e-book emphasize 3 elements of testicular mobilephone functionality: first, the molecular research of the phone cycle; moment, exam of the phone cycle, together with the functionality and id of particular macromolecules that direct the proliferation and differentiation of germ cells; and 3rd, the improvement of Leydig cells and the function of particular macromolecules within the formation of testicular steroids. every one bankruptcy relies on a lecture awarded on the XIIIth Testis paintings store hung on March 30 to April 1, 1995, on the Radisson Plaza inn in Raleigh, North Carolina. the choice of issues displays the recommenda tions of the workshop'S organizing committee. honest thank you are as a result of the audio system who agreed to lecture and get ready chapters.
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Extra info for Cellular and Molecular Regulation of Testicular Cells
The periods during which protein synthesis is required for the enactment of meiosis I, II, and the first mitotic division is indicated. Mos protein, which is not found in the oocyte, is synthesized following progesterone treatment and is maintained until the time of fertilization, when it is proteolyzed. FIGURE meiosis II following fertilization or the end of M phase during the mitotic divisions when the proteolysis of this protein appears to be more complete (4,5). During meiosis, additional cyclins are synthesized (cyclins AI, BI, and B2) as well.
5). The activity of endogenous sphingomyelinase in oocytes also was measured at successive times following progesterone treatment. This activity also rapidly increased (within 2 minutes) to a 3-fold level above that found in untreated oocytes (32). These results suggest that the increase in ceramide mass following progesterone treatment is due to the early activation of endogenous sphingomyelinase. At what point in the pathway of signaling events required for progesterone-induced oocyte activation and meiosis might ceramide be mediating its effects?
Following meiosis I, MPF is reactivated and entry into meiosis II is initiated, whereupon the still-condensed chromosomes align on the metaphase plate of the second meiotic spindle. At this point the oocyte has matured into the fertilizable gamete, the egg. The cell cycle arrests at this metaphase accompanied by high levels of MPF activity until the time of fertilization, whereupon cyclin B is proteolyzed, MPF is inactivated, meiosis II is completed to generate a second polar body, and the cell enters into S phase of the first mitotic cell cycle.