By Pietro Buzzini, Rosa Margesin
Yeasts are a flexible workforce of eukaryotic microorganisms, displaying heterogeneous dietary profiles and a rare skill to outlive in a variety of traditional and man-associated ecosystems, together with chilly habitats. Cold-adapted yeasts inhabit a variety of low-temperature environments the place they're subjected to seasonal or everlasting chilly stipulations. as a result, they've got developed a couple of edition recommendations in regards to progress and replica, metabolic actions, survival and safeguard. because of their exact skill to thrive effectively at low or even subzero temperatures, cold-adapted yeasts are more and more attracting consciousness in easy technology and for his or her huge, immense biotechnological potential.
This e-book provides our present realizing of the variety and ecology of cold-adapted yeasts in all over the world chilly ecosystems, their variation techniques, and their biotechnological value. distinct emphasis is put on the exploitation of cold-adapted yeasts as a resource of cold-active enzymes and biopolymers, in addition to their merits for foodstuff microbiology, bioremediation and biocontrol. additional, points of meals biodeterioration are considered.
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Additional resources for Cold-adapted Yeasts: Biodiversity, Adaptation Strategies and Biotechnological Significance
For example, a recent publication of yeasts isolated from a glacial lake includes descriptions of the elevation, slope, sun exposure, surface area, maximum depth, pH, dissolved solids (conductivity), Secchi disk transparency, summer air and water temperatures, and how many months per year the lake is frozen (Libkind et al. 2004). A Secchi disk is a black-and-white disk lowered into the water on a rope to determine water clarity, a property that may reflect nutrient levels in the water. Replicate samples are recommended if possible.
Many researchers prefer spread plates as they are simpler to perform, and it is easier to recover a yeast from a colony on the surface of the agar, rather than embedded in the agar. Furthermore, the warm temperature of the molten agar used in pour plates may be particularly injurious to cold-adapted yeasts. There are numerous nutrient agars available for cultivation of various types of yeasts (Beuchat 1998; Boundy-Mills 2006). ), and a vitamin source such as yeast extract or malt extract. Other supplements often include compounds such as rose Bengal or dichloran to inhibit or slow growth of filamentous fungi (Beuchat 1998) and antibiotics such as tetracycline or chloramphenicol to prevent growth of bacteria.
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