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By Prof. Harald zur Hausen (auth.)

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35 Distribution curves of the chromosome sets shown in Fig . 33 as present at the end of a flow cytometric analysis. n is the total number of registered chromosomes. The position and level of the peaks is characteristic for each cell line. The differences can be clearly seen Staff participating Monika Frank Gerry Futterman Prof. Dr. Klaus Goerttler Hans Peter Gotz 57 Fig. 36 A typical mitosis of the inoculated tumor (Walker 256 in the rat) investigated. The mitosis contained 63 chromosomes. The marker chromosome is indicated with an arrow.

Since this class of genes (and only this class) is read by type I RNA polymerase, antibodies against this polymerase provide a highly specific means of localizing transcriptionally active rRNA genes. By means of immunofluorescence microscopy and immune electron microscopy, we were able to show that the rRNA genes are located closely together in certain structural components (the "fibrillar centers") of the nucleoli. However, this typical spatial arrangement of the rRNA genes is abolished after experimental interference with maturation processes of the pre-rRNA.

Invasion and Metastasis 2, 313-360 (1982). Kerbel, R. , Lagarde, E. , Dennis, J. , Donaghue, T. : Spontaneous fusion between normal host and tumor cells: possible contribution to tumor progression and metastasis studied with a lectin resistant mutant tumor. Mol. Cell. Bioi. 3, 523-538 (1983). : Interaction of metastatic and non-metastatic tumor lines with aortic endothelial cell monolayer and their underlying basal lamina. In: Hormonally Defined Media. A Tool in Cell Biology (Fischer, Wieser, eds) Springer Verlag, Heidelberg, pp.

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