By Tanja Kostic, Patrick Butaye, Jacques Schrenzel
Written through major specialists within the box as a part of an interdisciplinary pan-European study application funded by way of the european, this ebook offers a special and finished review of ways microarray know-how can be utilized in competently monitoring the main hugely risky pathogens. a must have for public well-being enterprises occupied with bioterrorism in addition to all laboratories operating with BSL3 and/or BSL four brokers.
Read Online or Download Detection of Highly Dangerous Pathogens: Microarray Methods for BSL 3 and BSL 4 Agents PDF
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Additional info for Detection of Highly Dangerous Pathogens: Microarray Methods for BSL 3 and BSL 4 Agents
Fukushima, M. and Kawaguchi, R. (2003) Detection and identiﬁcation of Escherichia coli, Shigella, and Salmonella by microarrays using the gyrB gene. Biotechnol. , 83, 721–728. D. G. (2002) Radical-generating coordination complexes as tools for rapid and effective fragmentation and ﬂuorescent labeling of nucleic acids for microchip hybridization. Anal. , 311, 103–118. , Marsh, W. and Steel, A. (2004) Use of the DNA ﬂow-thru chip, a three-dimensional biochip, for typing and subtyping of inﬂuenza viruses.
The MAQC Consortiums main conclusions conﬁrm that, with careful experimental design and appropriate data transformation and analysis, microarray data can indeed be reproducible and comparable among different formats and laboratories, irrespective of sample labeling format. The data also demonstrate that fold change results from microarray experiments correlate closely with results from assays like quantitative reverse transcription PCR . However, different experimental setups require different validation approaches.
P. D. (2003) Light-directed 50 ! 30 synthesis of complex oligonucleotide microarrays. , 31, e35. , et al. (2000) Distinct types of diffuse large B-cell lymphoma identiﬁed by gene expression proﬁling. Nature, 403, 503–511. T. and SantaLucia, J. Jr, (1998) Nearest-neighbor thermodynamics of internal AÁC mismatches in DNA: sequence dependence and pH effects. Biochemistry, 37, 9435–9444. J. L. (2000) Rapid diagnosis of bacteremia by universal ampliﬁcation of 23S ribosomal DNA followed by hybridization to an oligonucleotide array.