By James R. Norris, David J. Read, A. K. Varma
Volumes 23 and 24 of this hugely acclaimed sequence specialize in tools used for the learn of either ectomycorrhiza and vesicular-arbuscular mycorrhiza. Written by way of a workforce of foreign specialists, those volumes contain the main broad compilation of equipment to be had in this subject.
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Extra info for Techniques for the Study of Mycorrhiza Part II
The relationships among single environmental factors or factor interactions and host plant growth and the extent of colonization in roots, the amount of extraradical mycelium, and the rate of propagule production must all be determined. Spore production is related to root colonization and percentage of colonization is proportional to the logarithm of the number of new spores produced (Furlan and Fortin, 1973; Wilson and Trinick, 1982). Other relationships also need to be quantified such as that between living mycelium in roots and the length of living extraradical mycelium, or the relationship between the number of propagules surviving in dried roots or as extraradical hyphae from dried roots and living mycelium in living roots.
This chapter is primarily concerned with methods for collecting data which need to be analysed rather than with experimental design, analysis in time and space, and modelling. The reader’s attention is drawn to the many statistical texts which discuss, for example, the initial steps in planning experiments; sampling procedures in inherently heterogenous environments which need to be assessed in the vertical and horizontal profiles; methods for increasing the accuracy of experiments such as determining sampling numbers to reduce errors; and factors involved in choosing time intervals between samplings versus the numbers of samples that can be processed by an operator.
TOMMERUP propagules that are a host-independent phase (Baylis, 1969). The methods for enumerating propagules for population biology overlap with those used for quantifying inoculum, whether the inoculum is being introduced to or already exists in a soil. A. Experimental design and sampling techniques Soil is a heterogeneous physical, chemical and microbiological environment and vesicular-arbuscular mycorrhizal fungi are one microbiological component. The fungi are biotrophs and, like other biotrophic fungi, several phases of their life-cycle are dependent on host plants for development.